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SPCI - Sociedade Portuguesa de Cuidados Intensivos

Revista Brasileira de Terapia Intensiva

AMIB - Associação de Medicina Intensiva Brasileira


ISSN: 0103-507X
Online ISSN: 1982-4335

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Duarte PAD, Venazzi A, Youssef NCM, Oliveira MC, Tannous LA, Duarte CB, et al. Pacientes com infecção por vírus A (H1N1) admitidos em unidades de terapia intensiva do Estado do Paraná, Brasil. Rev Bras Ter Intensiva. 2009;21(3):231-236



Original Article

Outcome of influenza A (H1N1) patients admitted to intensive care units in the Paraná state, Brazil

Pacientes com infecção por vírus A (H1N1) admitidos em unidades de terapia intensiva do Estado do Paraná, Brasil

Péricles Almeida Delfino DuarteI, Alisson VenazziII, Nazah Cherif Mohamad YoussefIII, Mirella Cristine de OliveiraIV, Luana Alves TannousV, César Barros DuarteVI, Cíntia Magalhães Carvalho GrionVII, Almir GermanoVIII, Paulo Marcelo SchiavettoIX, Alexandre Luiz de Gonzaga Pinho LinsX, Marcos Menezes Freitas CamposXI, Cecília Keiko MiúraXII, Carla Sakuma de Oliveira BredtXIII, Luiz Carlos TosoXIV, Álvaro Réa-NetoXV

IPhysician General Intensive Care, Unit Hospital São Lucas - FAG and Professor of Medicine at Universidade Estadual do Oeste do Paraná - UNIOESTE - Cascavel (PR), Brazil
IIMedical Graduation Student at Universidade Estadual do Oeste do Paraná - UNIOESTE - Cascavel (PR), Brazil
IIIPhysician of the Adult Intensive Care Unit, Hospital das Nações - Curitiba (PR), Brazil
IVPhysician of the Adult Intensive Care Unit, Hospital do Trabalhador - Curitiba (PR), Brazil
VPhysician of the Adult Intensive Care Unit Hospital Cajuru - Curitiba (PR), Brazil
VIPhysician of the Adult Intensive Care Unit - Hospital São Lucas - Curitiba (PR), Brazil
VIIPhysician of the Adult Intensive Care Unit Hospital Universitário Regional - Assistant Professor of the Intensive Care Discipline, Universidade Estadual de Londrina - UEL - Londrina (PR), Brazil
VIIIPhysician of the Adult Intensive Care Unit, Hospital Universitário - Maringá (PR), Brazil
IXPhysician of the Adult Intensive Care Unit, Santa Casa - Campo Mourão (PR), Brazil
XPhysician of the Adult Intensive Care Unit, Hospital Policlínica - Physician and Professor of Infectology at Universidade Estadual do Oeste do Paraná - UNIOESTE - Cascavel (PR), Brazil
XIPhysician, of the Adult Intensive Care Unit, Hospital Universitário do Oeste do Paraná's - Cascavel (PR), Brazil
XIIPhysician of the Adult Intensive Care Unit, Hospital Costa Cavalcanti's - Foz do Iguaçu (PR), Brazil
XIIIPhysician Residency Coordinator, Hospital Universitário do Oeste do Paraná's - Cascavel (PR), Brazil
XIVPhysician, of the Adult Intensive Care Unit, Hospital São Lucas - Faculdade Assis Gurgacz (FAG)-Cascavel (PR), Brazil
XVPhysician of the Intensive Care Study and Research Center - CEPETI and Medicine Professor at Universidade Federal do Paraná - Curitiba (PR), Brazil

Potential conflicts of interest: The authors reported no potential conflicts of interest.

Submitted September 15, 2009
Accepted September 22, 2009

Corresponding author:

Péricles Almeida Delfino Duarte
Hospital São Lucas/FAG's General ICU
Rua Engenheiro Rebouças, 2219
CEP: 85840.000 - Cascavel (PR), Brazil
E-mail: [email protected]



OBJECTIVE: This study aimed to analyze outcome, clinical and epidemiological characteristics and severity factors in adult patients admitted with a diagnosis of infection by virus A (H1N1) to public and private intensive care units, in Paraná, Brazil.
METHODS: Cohort study of medical charts of patients older than 12 years admitted to 11 intensive care units in 6 cities in the state of Parana, Brazil, during a period of 45 days, with diagnosis of swine influenza. The diagnosis of infection with A (H1N1) was made by real time polymerase chain reaction (RT-PCR) of nasopharyngeal secretion, or strong clinical suspicion when other causes had been ruled out (even with negative RT-PCR). Descriptive statistics were performed, analysis by the Chi square test was used to compare percentages and the Student's t test for continuous variables with univariate analysis, assuming a significance level of p <0.05.
RESULTS: There were 63 adult patients admitted with a diagnosis of H1N1, 37 (58.7%) being RT-PCR positive. Most patients were young adults (65% under 40 years of age) with no gender predominance and high incidence of obesity (27.0% with Body Mass Index > 30). Mean of the Acute Physiologic Chronic Health Evaluation II (APACHE II) score was 15.0 + 8.1. Mortality in the intensive care unit was 39.7%. The main factors associated with mortality were: positive RT-PCR, low levels of initial PaO2/FiO2, high initial levels of urea and lactate dehydrogenase, required level of positive end expiratory pressure, need for the prone position and vasopressors.
CONCLUSIONS: Adult patients with A (H1N1) virus infection admitted to intensive care units had a high risk of death, particularly due to respiratory impairment. Positive RT-PCR, urea and lactic dehydrogenase, low initial PaO2/FiO2 and high levels of PEEP were correlated with higher mortality.

Keywords: Influenza A virus; Intensive care units; Respiration, artificial.




In March-April 2009,cases of acute respiratory syndrome associated to influenza were described in Mexico and the United States and identified as a new swine influenza A virus.(1) This was a new virus, genotipically different from other A viruses: avian H1N1, swine or human (responsible for the Spanish Influenza in 1918). It quickly spread through the North Hemisphere within the next weeks, reaching Europe in May.

On June 11, 2009 the World Health Organization (WHO) raised the influenza A: H1N1 epidemic level to the maximum alert level of 6, officially declaring the world in a new influenza pandemic, which was considered "uncontrollable".(2)

The H1N1 pandemic reached Parana, Brazil early in June 2009. Shortly after, Parana became one of the states with the highest incidence and mortality rates of the disease in Brazil, apparently because of its frontier with Argentina, a country that along with Chile had the first South American H1N1 cases in May (end of fall in the Southern hemisphere).

This study aimed to observe and analyze Influenza A: H1N1 cases in patients aged above 12 years admitted to 11 Intensive Care Units (ICU) in 6 different cities in the Parana State, Brazil, for 45 days.



This was a cohort observational study with all patients above 12 years of age diagnosed with acute A: H1N1 virus infection admitted to an adult ICU in 11 hospitals of 6 cities in the state of Parana (Southern Brazil) evaluated from July 18, 2009 to August 21, 2009.

Patients' medical records and clinical epidemiologic information, plus ICU admission laboratory tests, oxygen therapy and mechanic ventilation data, along with ICU and hospital course were evaluated.

The Influenza A:H1N1 diagnostic testing was performed by the Real Time Polymerase Chain Reaction (RT-PCR) using oropharyngeal secretion - "Kit Superscript III Platinum One-Step Quantitative RT-PCR System"® (Invitrogen, Carlsbad, USA).

This test was performed at the Parana State Health Secretariat Central Laboratory. The inclusion criteria were: (1) patients with RT-PCR virological diagnosis or (2) patients clinically diagnosed (based on clinical-epidemiologic data), however with a negative RT-PCR test. In RT-PCR negative cases, clinical diagnosis was reached by excluding other conditions such as seasonal influenza and other viruses, or bacterial pneumonia.

Descriptive statistics and Chi square percentual comparisons were performed, and the quantitative variables were compared using the Student's t test, with a p<0.05 significance level. Analysis was univariated. The study was approved by the Universidade Estadual do Oeste do Paraná Ethics Committee.



During the study period there were 574 admissions in the 11 studied ICUs (55.7% in private hospitals and 44.3% in public or philanthropic hospitals), of which 63 patients (11.0%) with influenza H1N1 infection were included. The patients' epidemiological data are shown on table 1. The mean age was 35.0 years, and 46.1% were male. Of the H1N1 patients, 42.9% were treated in private ICUs, and 57.1% in public ICUs. The mean time from the onset of symptoms to ICU admission was 6.0 days. All patients underwent RT-PCR nasopharyngeal secretion testing, which was positive in 37 (58.7%) patients. Invasive mechanic ventilation was required for 71.4%.

The admission laboratory tests and clinical features are shown in table 2.

The ICU mortality was 39.7%, while of those under mechanic ventilation (MV) it was 53.3%. There was no difference in mortality between private and public ICUs (36.1% vs 44.4%, p=0.685).

Eight of the patients were pregnant (12.7%), including one in the 3rd post-partum day. Maternal mortality in the ICU was of 25%.

There was no age-related difference in mortality (x2=5.09; p=0.16).

The main ICU mortality related factors (Table 3) were positive RT-PCR test, initially low arterial oxygen pressure/inspired oxygen fraction rate (PaO2/FiO2), initially increased urea and lactic dehydrogenase (LDH), positive end-expiratory pressure (PEEP) required , need for prone position and use of vasopressor drugs.

Table 4 displays the outcomes of positive and negative RT-PCR results.



In March 1918 (during World War I) a severe influenza epidemic spread throughout the world, beginning simultaneously in the U.S. and Europe and killing (particularly in the "second wave", that started 5 months later) about 40 million people, becoming the most catastrophic medical event ever in human history.(3) The strain involved was Influenza A (H1N1) from birds. Since then, the influenza virus surveillance systems had identified changes in the A virus epidemic strains every 1 to 2 years (by surface glycoprotein mutations, hemagglutinin and neuropeptidase). In the last 91 years, two A virus pandemics were identified: in 1957 (H2N2) and 1968 (H3N2), although they were much less lethal.(4-6) During the last years, with epidemics observed such as severe acute respiratory syndrome (SARS), the imminence of an influenza pandemic has been alerted by the health authorities.(3,7,8)

In this trial, it was seen that 11.0% of ICU admissions were due to H1N1 cases. An important bias is that these hospitals were mostly considered as reference for treatment of H1N1 patients, which could increase disease incidence. Another important factor to bear in mind is that in, at least one hospital, a special unit was created for H1N1 patients. Thus, in practice the number of ICU beds increased during this period (as the original ICU continued to take care of non-H1N1 patients). Finally, there were instructions to reduce the elective surgeries in public hospitals as a health system strategy, which could temporarily reduce some indications for ICU admissions.

The ICU mortality in this study was high, particularly among invasive MV patients. This finding is also found in literature. In 30 of the admitted cases (either with virological or clinical diagnosis) in California hospitals (USA), six patients were admitted to the ICU (4 under mechanic ventilation), with no death (although three were still hospitalized at the reporting time).(9)According to Perez-Padilha et al.,(10) of the 18 H1N1 cases confirmed in patients with respiratory failure, 10 under mechanical ventilation were reported; of the total of patients under mechanical ventilation (MV), 70% died.

The main mortality risk factors identified were related to clinical severity, particularly respiratory impairment. Another trial found these main factors associated to higher risk of mortality: hypotension requiring vasoactive drugs, acute renal failure, metabolic acidosis, APACHE II, PaO2/FiO2 and Sequential Organ Failure Assessment (SOFA) admission score.(10) The severe respiratory impairment is similar to severe influenza cases, just as SARS.(11)

As previously described in literature,(10) ICU mortality was higher among positive RT-PCR test patients than among those with a negative test. This could reflect a higher viral load and morbidity in positive RT-PCR patients, although as mentioned below, this could also mean that among the RT-PCR negative patients other diagnoses, with milder courses could be included. Another possibility is inappropriate material collection, which could reflect in increased false-negatives rates.(12)

Pregnancy is acknowledged as a risk factor for respiratory complications in infections by influenza.(13,14) Among the factors justifying the high incidence in this group, physiological changes of pregnancy, including reduced pulmonary functional residual capacity and cell-mediated immunity impairment, are emphasized.(15) However, in patients with severe infection requiring ICU stay, the mortality rate is similar to that of the general population.(9) In our study the maternal mortality among pregnant women was similar to the overall group (25.0% vs 41.8%; p=0.602).

Obesity has clearly been incriminated as a severity and mortality risk factor among swine influenza patients.(16) It is believed that this is due both to the disease-related respiratory effects (reduced pulmonary functional residual capacity) and presence of typically associated comorbidities (such as diabetes, asthma, cardiovascular diseases, etc).(9) Obesity was very frequent among this group of patients, similar to literature: 27.0% had a body mass index (BMI) above 30.

The time from onset of the symptoms and ICU admission was relatively long (6 days). In the Spanish trial,(16) time from onset of symptoms and beginning of treatment was 4 days.

This study has limitations. The total number of cases may have been overestimated. In our study, were considered as H1N1 cases, not only patients with confirmed laboratory diagnosis, but also those with strong clinical-epidemiological suspicion, although with negative RT-PCR. According to the Health Ministry of Brazil,(17) "influenza A:H1N1 will be discarded if influenza A virus is not detected by RT-PCR or culture techniques." However, although RT-PCR is the World Health Organization (WHO) and Center for Disease Control (CDC) recommended method,(12,18) false-negative results reach 10%.(19,20) Thus, the investigators decided to assess the strongly suspected cases (clinical diagnosis), even though with a negative influenza A test (since other causes were discarded). This was the same methodology as that used in previous reports,(9,10) although in the Spanish trial(16) only positive cases were accepted for diagnosis. However, the authors recognize that this approach constitutes a significant limitation for this study regarding overall data interpretation.

The univariate analysis may have been a restricted tool to define mortality-associated risk factors and therefore a limitation for interpretation of this study data.



Adult patients with an influenza A(H1N1) diagnosis admitted to an ICU have an increased mortality rate. The main mortality predictive factors found in this trial were: severe respiratory impairment, positive RT-PCR test, increased baseline urea and LDH, and need for vasopressor drugs. However, diagnostic methods (including clinical diagnosis) and the reduced number of patients may have contributed to jeopardize interpretation of this study data.



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14. Centers for Disease Control and Prevention (CDC). Novel influenza A (H1N1) virus infections in three pregnant women - United States, April-May 2009. MMWR Morb Mortal Wkly Rep. 2009;58(18):497-500. Erratum in: MMWR Morb Mortal Wkly Rep. 2009;58(19):541.

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Received from the Hospital São Lucas - Cascavel (PR), Brazil.



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